The indentifacation fof prevotella intermedia by conventional methods in difficult,time consuming,lacking of specifcicity or sensitivity and sometime unreliable. Therefore,polymerase chain reaction(PCR)methods for detection of P.intermedia were developed for rapid and easy indentification of this period on topathogen in subgengival plaque samples. This study demonstrates the utility of PCR detectuin method for indentifying P.intermedia and indicates a strong association between P.intermedia and adult periodontitis.